Laboratory Research

Patients of both groups underwent the following laboratory studies before and after therapy: clinical blood test, a coagulogram (fibrinogen, prothrombin time, D-dimer), blood biochemical composition (C-reactive protein, glucose, ferritin), and an immunogram (CD3, CD4, CD8, interleukin-6). For the study, fasting blood test was taken by venipuncture from the cubital vein into special vacuum tubes. The collection of biological material was carried out completely in accordance with the requirements of the preanalytical stage of the hematological study. Laboratory studies were performed using reagents from the following manufacturers: hematological studies and determination of CD3, CD4, CD8 lymphocytes were done using an automatic hematological analyzer Cell-Dyn Sapphire, manufactured by Abbott Laboratories (USA); ESR measurement was done on the Test 1 analyzer manufactured by Alifax (Italy); coagulogram was analyzed on an automatic coagulometer ACL9000, manufactured by Instrumentation Laboratory (USA); the level of glucose and C-reactive protein was determined using a biochemical analyzer ARCHITECT c4000, manufactured by Abbott Laboratories (USA); ferritin concentration was measured on an ARCHITECT i2000 immunochemiluminescence analyzer, manufactured by Abbott Laboratories (USA); and the level of interleukin-6 was determined on equipment designed for ELISA, with reagents from the “Vector-Best” company.