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A total of 5,000 A549, H1299, or LLC cells were seeded in each well of a 96-well microplate with 100 μL of the cell culture medium. Different concentrations of the vehicle or M3G were added to the wells and co-incubated for 24, 48, and 72 h in a CO2 incubator. The Cell Counting Kit-8 (CCK-8) reagent (Dojindo, Kumamoto, Japan) was added (10 μL) into each well and further incubated for 1–2 h. The absorbance was measured at 450 nm using a microplate reader. Each experiment was conducted three times.
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