ELISA

JW Jie-Min Wang
YL Yong-Jiang Li
JW Jun-Yong Wu
JC Jia-Xin Cai
JW Jing Wen
DX Da-Xiong Xiang
XH Xiong-Bin Hu
WL Wen-Qun Li
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The level of CD63, CD81, TSG101, beta-actin, GAPDH, CD47 (CUSABIO Biotech Co. Ltd., China) and ago-1 (MyBiosource, Canada) in purified samples and in crude sEVs were determined by ELISA kits. The protein per EV was calculated by the following formula. The assay was repeated 3 times.

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