En face Oil Red O (ORO) staining

XZ Xinxin Zhang
YQ Yating Qin
XW Xiaoning Wan
HL Hao Liu
CL Chao Lv
WR Weibin Ruan
LH Lin He
LL Li Lu
XG Xiaomei Guo
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En face plaque area was commonly examined by ORO (ORO, St. Louis, USA) staining of lipid deposits. The entire aorta was dissected from mice free of fat and connective tissues, then unfolded, opened longitudinally and soaked in 4% paraformaldehyde overnight. Subsequently, the whole aorta was stained with 0.5% ORO solution and photographed with a digital camera (Olympus Corporation, Japan).

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