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We performed the cell cycle analysis using a flow cytometric technique, as previously described (34,35). Briefly, we stained cells with propidium iodide (PI) (MP Biomedicals, LLC) and analyzed the cells using a BD FACSCalibur Flow Cytometer, with Cellquest Pro software (BD Biosciences). We measured 10,000 events of the gated population. We identified the percentage of cell distributions in the various cell cycle phases using ModFit LT 5.0 (Verity Software House).
Copyright and License information: ©2021 Hossian et al.
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