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ATP contents in the primary astrocytes of the WT and kir6.2−/− mice were detected using an Enhanced ATP Assay Kit (Beyotime, S0027) according to the manufacturer’s protocol. Cells were lysed in an ATP lysis buffer and the amount of protein in each sample was homogenized with a lysis buffer after protein quantification. The ATP contents were determined using a luminometer.
Copyright and License information: ©2021 Song, Zhu, Xu, Liu, Fang, Zhang, Ding, Hu and Lu.
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