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Polyethylene glycol (PEG) was used to decrease the solubility of origami and induce origami precipitation39. Origami solution in 1× TE buffer with 12.5 mM MgCl2 was mixed 1:1 with 15% PEG buffer (7.5 g PEG-8000, 1× TAE, 12.5 mM MgCl2, 500 mM NaCl) and centrifuged at 20,000 × g at 4 °C for 30 min. The supernatant was removed and origami was resuspended in folding buffer (12.5 mM MgCl2, 10 mM Tris, 1 mM EDTA at pH 8.0). Centrifugation and supernatant removal were repeated three times. Origami was stored at −20 °C.
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