Standardized Skin Surface Biopsy

An SSSB sample was taken from the same place from which the dermoscopic image was taken. An area of 1 cm² was marked on a microscope slide. A drop of cyanoacrylate was placed on the other side of the slide in the middle of this area. The sample was gently pressed onto the surface and removed slowly after about 30–45 seconds. A few drops of glycerin were dropped onto the biopsy specimens and covered with coverslip. The samples were examined with a light microscope (Leica DM750, Switzerland) at ×4, ×10, and ×40 magnifications by an expert parasitologist. The diagnosis of 5 or more Demodex mites in 1 cm² was evaluated as positive. Species identification of mites was made in accordance with the relevant literature [2].