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Total RNA was extracted from snap-frozen tissues using RNX-Plus (CinnaGen, Iran) according to the manufacturer’s instruction. DNase treatment was performed using Fermentas DNase I (Fermentas, Lithuania) to eliminate genomic DNA. cDNA synthesis was performed with random hexamers and oligo-dT primers using First Strand cDNA Synthesis Kit (Fermentas, Lithuania).
Copyright and License information: ©2021 Asadi et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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