NP-KLH immunization

LL Limin Liu
LH Longyuan Hu
LY Linxuan Yang
SJ Sujie Jia
PD Pei Du
XM Xiaoli Min
JW Jiali Wu
HW Haijing Wu
HL Hai Long
QL Qianjin Lu
MZ Ming Zhao
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Age-matched mice were immunized with NP-KLH (Bioresearch Technologies, USA) emulsified in Imject™ Alum Adjuvant (Thermo Fisher, USA) that was administered to each mouse by subcutaneous injection. After immunization for one week, we injected the same amount of NP-LKH to boost mouse immunity for another week. Then, we collected the spleen, lymph nodes and serum for subsequent experiments.

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