Lysyl Oxidase Activity Assay

QG Qing-Yuan Gao
HZ Hai-Feng Zhang
JT Jun Tao
ZC Zhi-Teng Chen
CL Chi-Yu Liu
WL Wen-Hao Liu
MW Mao-Xiong Wu
WY Wen-Yao Yin
GG Guang-Hao Gao
YX Yong Xie
YY Ying Yang
PL Pin-Ming Liu
JW Jing-Feng Wang
YC Yang-Xin Chen
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Lysyl oxidase (LOX) activity was measured using the Lysyl Oxidase Activity Assay Kit (Abcam, Cambridge, UK) following the manufacturer's instructions. Briefly, cell culture supernatants were collected and incubated for 30 min in the presence of 50 μl of the reaction mixture. Fluorescence was monitored with a fluorescence plate reader at 540/590 nm excitation/emission wavelengths.

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