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Homogenization of Brain Tissue

CV Christina Veit
AJ Andrew M. Janczak
BR Birgit Ranheim
JV Judit Vas
AV Anna Valros
DS Dale A. Sandercock
PP Petteri Piepponen
DD Daniela Dulgheriu
JN Janicke Nordgreen
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The frozen brain tissue blocks were mechanically homogenized using a pestle and a mortar that was placed on dry ice and filled repeatedly with liquid nitrogen to keep the sample frozen. The pulverized brain tissue was transferred into 2-ml round-bottomed Eppendorf tubes and weighed. Ten tubes with tissue from the frontal cortex (five left and five right), hippocampus (five left and five right), and brain stem (five left and five right), respectively, and six tubes with tissue from the hypothalamus (three left and three right) were collected and stored at −80°C until analysis.

Copyright and License information:  ©2021 Veit, Janczak, Ranheim, Vas, Valros, Sandercock, Piepponen, Dulgheriu and Nordgreen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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