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Photoacoustic Imaging

HD Hélène David
AU Aurore Ughetto
PG Philippe Gaudard
MP Maëlle Plawecki
NP Nitchawat Paiyabhroma
EZ Emma Zub
PC Pascal Colson
SR Sylvain Richard
NM Nicola Marchi
PS Pierre Sicard
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The Vevo LAZR-X (VisualSonics/Fujifilm) system was used in this study to generate the photoacoustic (PA) images in association with Vevo3100. PA images were obtained at 750- and 850-nm excitation wavelengths, with the array transducer of 20 MHz central frequency (MX250), due to maximal absorption of oxy- and deoxyhemoglobin at these wavelengths using the oxy-hemo mode to determine sO2. For the PA signal acquisition, the gain was set to 34 dB with 2D gain of 22 dB. A B-mode was also performed for colocalization of photoacoustic signals. Measurements were realized at 4 h, 1 day, and 7 days after surgery. In order to increase temporal and spatial resolution for myocardial anterior wall sO2 measurement, we combined PA imaging with ECG-gated kilohertz visualization. Calculation of sO2 was achieved offline with the VevoLAB software by selecting areas of interest (myocardial anterior wall, brain, liver, and kidney) in B-mode.

Copyright and License information:  ©2021 David, Ughetto, Gaudard, Plawecki, Paiyabhroma, Zub, Colson, Richard, Marchi and Sicard.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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