2.12. Generation of GLAST::CreERT2/Gdi1flox mice

Heterozygote Gdi1^lox/X female mice [8] were crossed with transgenic GLAST::CreERT2 male mice [23] (obtained from M. Götz, Munich, Germany). Mice genotype was analysed by PCR analysis of DNA extracted from the tail using primers Lox1 (5′ GGA AGA CTT GGA AGC TGA AAG CTT T 3′) and Lox2 (5′ CAT GAT GCC AGA CAG GAT GCA TTC 3′), GLAST F8 (5′ GAG GCA CTT GGC TAG GCT CTG AGG A 3′), GLAST R3 (5′ GAG GAG ATC CTG ACC GAT CAG TTG G 3′) and GLAST CER1 (5′ GGT GTA CGG TCA GTA AAT TGG ACA T 3′) as previously described[8; 23]. All animals were maintained on a 12 h light/dark cycle at 22–25 °C. Food pellets and water were available ad libitum.