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Advanced Glycation End-Product Competitive ELISA
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An Advanced glycation end-product (AGE-BSA) competitive ELISA kit (cell biolabs cat no. STA-817) was used. Fifty microliter of plasma from each individual per species was plated in duplicate and an estimation of the concentration of AGE-BSA in plasma samples based on an AGE-BSA standard curve was performed, as described by the manufacturer. Plates were read at 450 nm using a TECAN infinite m200 plate reader. Each individual's AGEs-BSA concentration can be found in Supplementary Table 1.
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