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5.10. Germline LOH
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Point mutations classified as germline from VarScan2 (2.3.6) were obtained from WGS data for both samples. Mutations were then binned across chromosomes such that each bin corresponds to an approximately 500,000 base pair window and the frequency of mutations in each bin was calculated. Genomic regions where a bin exhibited a mutational frequency less than 5 were considered as LOH owing to the decrease in frequency of heterozygous single nucleotide polymorphisms (SNPs) in that area.
Copyright and License information: ©2021 Chu et al
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