CITE-seq antibody staining

TT Thomas J. Tull
MP Michael J. Pitcher
WG William Guesdon
JS Jacqueline H.Y. Siu
CL Cristina Lebrero-Fernández
YZ Yuan Zhao
NP Nedyalko Petrov
SH Susanne Heck
RE Richard Ellis
PD Pawan Dhami
UK Ulrich D. Kadolsky
MK Michelle Kleeman
YK Yogesh Kamra
DF David J. Fear
SJ Susan John
WJ Wayel Jassem
RG Richard W. Groves
JS Jeremy D. Sanderson
MR Michael D. Robson
DD David P. D’Cruz
MB Mats Bemark
JS Jo Spencer
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Cryopreserved samples were thawed and sorted using the gating strategy in Fig. S3 A. Cells were then washed and stained in a CITE-seq antibody cocktail at a concentration of 8 µg/ml for 30 min on ice (Fig. S3 B). Cells were then washed three times before loading onto the 10x Chromium controller.

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