Colony Formation Assay

RY Rui Yu
YL Yefen Lu
RY Ren Yu
JX Jianjun Xie
SZ Shoujun Zhou
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For colony formation assay, cells were seeded into 6-well plates at a density of 200 cells/well. Then cells were exposed to different treatment for two weeks, cells were fixed with methanol and staining with 0.1% crystal violet and the number of colonies were recorded under inverted microscope (Olympus, Tokyo, Japan).

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