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Colorectal cancer cells were seeded in 96‐well plates. Adherent cells were incubated with various concentrations of compounds. HCT116 cells (3 × 103 per well) and Lovo cells (3 × 103 per well) were treated for 1 ‐ 5 days, two patient‐derived cell lines (6 × 103 per well) were treated for 72 h. Cell viability was measured according to the instruction of an MTS assay kit (Promega). The half inhibition concentration (IC50) value was calculated using GraphPad Prism 8 software.
Copyright and License information: ©2020 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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