4.6. m6A RNA Methylation Quantification

To determine m⁶A RNA methylation status from hippocampal tissue, we used the EpiQuik™ m⁶A Quantification Kit according to the manufacturer’s instructions (Epigentek, Farmingdale, NY, USA) using total RNA isolated from mice. The kit is based on specific antibody detection of m⁶A residues, which trigger an ELISA-like reaction that allows colorimetric quantification by reading absorbance at 450 nm using a Microplate Photometer. The absolute amount of m⁶A (proportional to the Optical Density [OD] intensity) was measured and quantified using a standard curve plotting OD values vs. six serial dilutions of a control m⁶A (0.01–0.5 ng).