4.2. The Screen of Antifungal Bacteria

The fungus as indicated was cultivated on a PDA plate (0.4% potato infusion powder, 2% glucose, 1.5% agar) at 28 °C for 4 days. A 1-cm-diameter agar plug, full of mycelium, was cut out from the 4-day-cultured fungal colony and positioned onto the center of another plate of PDA, KBA (2% peptone, 0.15% K₂HPO₄, 0.15% MgSO₄·7H₂O, 1.5% glycerol, 1.5% agar, pH 7.2) or LBA (1% tryptone, 0.5% yeast extract, 1% NaCl, 1.5% agar). The fungus was cultivated continuously until the diameter of the radial colony reached about 6 cm in diameter. Subsequently, a 200-µL aliquot of the overnight culture of screened microorganisms was dropped onto a 0.6-cm-diameter filter paper, placed at a distance of 0.5 cm to the periphery of the fungal colony. The plate was incubated continuously at 28 °C, and the growth of the pathogenic fungus confronted by screened microorganisms was recorded daily. The incubator used in this study was made by Yih Der Technology Co., (New Taipei City, Taiwan).