Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Peptide mass fingerprinting (PMF)

MC Mario Carrasco
PV Pablo Villarreal
SB Salvador Barahona
JA Jennifer Alcaíno
VC Víctor Cifuentes
MB Marcelo Baeza
request Request a Protocol
ask Ask a question
Favorite

Processing and analysis of protein samples were performed at the Central Service for Experimental Research (Valencia, Spain). Samples were digested with sequencing grade trypsin (Madison, WI 53711 USA) as described previously [48]. The digestion reactions were stopped with TFA (1 % final concentration) and then concentrated to 20 μL by speed vacuum to analyze 5 μL by liquid chromatography and tandem mass spectrometry (LC–MS/MS). The obtained data were analyzed by Mascot, and only the results with a score greater than 54 (P < 0.05) were considered statistically significant [49].

Copyright and License information: Carrasco et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A