All iPSCs were cultured on hESC-qualified Corning® Matrigel® (Corning #354277, Corning, NY, USA) in mTeSRTM1 (STEMCELL Technologies #85850, Vancouver, BC, Canada). Reprogramming of BBS1-iPSC is described in Reference [44], BBS5 iPSC in Reference [46] and BBS10-iPSC in Reference [45].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.