3.6.3. Cytotoxicity Calculation via MTT Assay

After 24 h of treatment with different concentrations of compounds 5 and 6, cells were washed with phosphate buffer saline (PBS) (Invitrogen Inc., Carlsbad, CA, USA), and then incubated with 100 µL of DMEM and 25 µL MTT solution (Invitrogen Inc.) for 3–4 h. After 4 h formazan crystals were solubilized with 10% sodium dodecylsulphate (SDS) (Invitrogen Inc.) and absorbance was taken at 570 nm [76]. Percentage viability was calculated according to the following formula:

where abs stands for absorbance.

Experiments were repeated three times for the average calculations.