3.4. Preparation of Antibody-Nanoparticle Conjugates with AuNPs, AgNPs and EuNPs

Commercially available kits were used for the preparation of conjugates with gold, silver and europium nanoparticles. All necessary reagents, such as antibody diluent, reaction buffer and quencher, were supplied as part of the package. Antibody binding was performed according to the manufacturer’s protocol. For 10 nm AgNPs, the antibody was diluted with Antibody diluent to the concentration of 0.5 mg/mL compared to the recommended 3 mg/mL because such a concentrated antibody was not available. The manufacturer’s instructions were followed.