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Scratch Wound Migration Assay

WC William Cathery
AF Ashton Faulkner
EJ Eva Jover
IR Iker Rodriguez-Arabaolaza
AT Anita C. Thomas
EA Elisa Avolio
MC Massimo Caputo
PM Paolo Madeddu
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Confluent HUVEC monolayers were seeded in 0.5% gelatin-coated 96 well-plates (15,000 cells/cm2) in EGM-2. A scratch was made using a 10 μl pipette tip. HUVECs were then incubated for 12 h with CM or control medium, which were supplemented with 2 mM hydroxyurea (Sigma-Aldrich) to inhibit proliferation. HUVECs were imaged at 0 and 12 h using a Leica DMi1 inverted microscope and the scratch area quantified using ImageJ.

Copyright and License information:  ©2021 Cathery, Faulkner, Jover, Rodriguez-Arabaolaza, Thomas, Avolio, Caputo and Madeddu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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