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Total RNA was extracted from the heart samples or cultured cardiomyocytes. Reverse transcriptional reactions were performed using the PrimeScript RT Reagent Kit (#RR036B, TaKaRa, Otsu, Japan). SYBR Green PCR Master Mix (04887352001, Roche) was used to detect the PCR amplification products. The mRNA levels of the target genes were normalized to those of GAPDH. The levels of miR-152 were detected using an All-in-One™ miRNA qPCR Kit (Genecopia).
Copyright and License information: ©2021 Wen-Bin Zhang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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