RNA purification using the Qiagen RNeasy kit

TG Thomas G. W. Graham
CD Claire Dugast-Darzacq
GD Gina M. Dailey
XN Xammy H. Nguyenla
ED Erik Van Dis
ME Meagan N. Esbin
AA Abrar Abidi
SS Sarah A. Stanley
XD Xavier Darzacq
RT Robert Tjian
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RNA was purified from 100 μL of each swab sample using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, Cat. #74136). 600 μL of buffer RLT was used in the first step, and RNA was eluted with 50 μL of water in the final step. The gDNA eliminator column step was omitted.

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