Oxygen Glucose Deprivation (OGD) Model

Oxygen glucose deprivation (OGD) model is widely used as a feasible in vitro model for ischemic stroke (Yang et al., 2019; Poupon-Bejuit et al., 2020). Hence, the OGD model was applied to examine the underlying potential cellular mechanisms in the present study. For OGD, the DMEM/F12 medium was replaced with glucose-free DMEM medium. Then, NSCs were incubated in a well-characterized and finely controlled ProOx-C-chamber system (Biospherix, Redfield, NY) with decreased O₂ and increased N₂ levels under humidified 5% CO₂ condition at 37°C for 8 h. The O₂ concentration in the chamber was controlled by the ProOx model 110 and maintained at 1%. For control group, NSCs were subjected to the same procedures as the OGD groups except that they were exposed to 21% O₂ and normal glucose condition during the entire duration.