Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

BV2 cell line culture protocol and stimulation

WL Weifen Li
TA Tahir Ali
CZ Chengyou Zheng
ZL Zizhen Liu
KH Kaiwu He
FS Fawad Ali Shah
QR Qingguo Ren
SR Shafiq Ur Rahman
NL Ningning Li
ZY Zhi-Jian Yu
SL Shupeng Li
request Request a Protocol
ask Ask a question
Favorite

Mouse microglial BV2 cell lines were grown in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Gibco, Waltham, MA). The cells were maintained in a humidified incubator with 95% air and a 5% CO2 atmosphere at 37 °C. Medium containing the appropriate agents was replaced every other day. When the cells grew to a density of about 90%, exifone was added to the cell medium, fluoxetine was added to the cell culture after 16 h, lipopolysaccharide was added after 2 h, and cells were harvested after 4 h.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A