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BV2 cell line culture protocol and stimulation
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Mouse microglial BV2 cell lines were grown in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Gibco, Waltham, MA). The cells were maintained in a humidified incubator with 95% air and a 5% CO2 atmosphere at 37 °C. Medium containing the appropriate agents was replaced every other day. When the cells grew to a density of about 90%, exifone was added to the cell medium, fluoxetine was added to the cell culture after 16 h, lipopolysaccharide was added after 2 h, and cells were harvested after 4 h.
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