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To the dried extracts, add dilute HCL dropwise and filter it. The resulting filtrate was screened for the preliminary tests of alkaloids.
Test with Dragendorff's reagent: The reagent was mixed with the filtrate on a watch glass to observe orange, brown precipitates that reveal the alkaloids' occurrence.
Test with Mayer's reagent: The reagent was mixed with the filtrate on a watch glass to observe cream-colored precipitates that reveal the alkaloids' occurrence.
Test with Wagner's reagent: The reagent was mixed with the filtrate on a watch glass to observe reddish-brown precipitates that reveal the alkaloids' occurrence.
Test with Hager's reagent: The reagent was mixed with the filtrate on a watch glass to observe yellow precipitates that reveal the alkaloids' occurrence.
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