Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Differential cell aggregation assay
Ask a
question
Favorite
To reveal differential adhesion between cell populations, we used the hanging drop aggregation assay with two cell populations separately labeled with green or red fluorescent dyes36. For cell labeling, dissociated cells were incubated in 5 µM CellTracker dye for 30 min, suing CMFDA (Invitrogen C7025) for green and CMTPX (Invitrogen C34552) for red labeling. In total, 1 × 104 green cells and 1 × 104 red cells were mixed evenly before seeding in each droplet. Live-cell images were taken after 24 h of incubation with an inverted Nikon fluorescent microscope.
Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
