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Gel autoradiography

TM Takahiro Morito
RH Ryuichi Harada
RI Ren Iwata
YD Yiqing Du
NO Nobuyuki Okamura
YK Yukitsuka Kudo
KY Kazuhiko Yanai
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The radiochemical purity of the product was confirmed by SDS-PAGE based on the NuPAGE system (Invitrogen). To estimate the molecular weight of the products, Novex Sharp Pre-stained Protein Standard (Invitrogen) was loaded onto the first lane of each gel, and radioactive liquid was spotted on a fixed thin layer chromatography plate at the position corresponding to 10, 20, and 40 kDa standard bands. The gel was placed in contact with a BAS-IP TR 2025 imaging plate (Cytiva) overnight, and autoradiographic images were acquired using a Typhoon FLA 9500 laser scanner (Cytiva). The images were analysed using ImageQuant TL (Cytiva).

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