2.8 Gnrh treatment of primary pituitary cell culture

Gnrh1 (Bachem, Budendorf, Switzerland) was added to the growth medium one day after plating the cells, to give a final concentration of 10⁻⁷ M. This is a concentration used previously in studies on both teleosts [19, 32] and mammals [24, 25] and shown to elicit robust responses. The cells were exposed to Gnrh1 and followed in time-lapses for up to 20 h. The experiments were performed from 8 independent cultures and the control cells were incubated in growth medium for similar time interval as the Gnrh1-exposed cells.