H9C2 cardiomyocytes (Boster Biological Technology, Wuhan, China) were cultured in DMEM medium containing 10% serum (GIBCO, USA) and 1% penicillin-streptomycin at 37 °C and 5% CO2 incubator. According to the experimental group, the original complete culture medium was replaced by serum-free low-sugar DMEM medium, and H9C2 cardiomyocytes were placed in an anoxic incubator with 37 °C, 5% CO2, and 2% O2 for 12 h. After hypoxia, the culture medium was replaced by complete culture medium and reoxygenated in an incubator of 37 °C and 5% CO2 for 12 h to establish a cell HR model.
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