Cox Regression Analysis and Kaplan-Meier Survival Analysis

Cox regression analysis was used to evaluate the relationship between SLC41A3 expression and overall survival and disease-specific survival of patients using the TCGA databases. The Kaplan-Meier method was used to assess the difference between “high” and “low” risk groups based on the best separation of SLC41A3 expression, employing R packages of survminer and survival. The “surv-cutpoint” function in the survminer R package was performed to search the best split by verifying all potential cut points. Subsequently, the patients were divided into high and low SLC41A3 expression groups with the maximum-selected log-rank statistic. Log-rank P-value, hazard ratio (HR), and 95% confidence intervals were examined.