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APEX proximity labeling and TEM.

EE Elin Einarsson
ÁÁ Ásgeir Ástvaldsson
KH Kjell Hultenby
JA Jan O. Andersson
SS Staffan G. Svärd
JJ Jon Jerlström-Hultqvist
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Cells were grown in LYI medium supplemented with 100 µM hemin (H9039; Sigma-Aldrich). Cells were fixed, DAB labeled, and processed for TEM as described in reference 25. The pellets were then postfixed in 2% osmium tetroxide in 0.1 M phosphate buffer, pH 7.4, at 4°C for 2 h; dehydrated in ethanol, followed by acetone; and embedded in LX-112 (Ladd, Burlington, Vermont). Ultrathin sections (approximately 50 to 60 nm) were cut by a Leica Ultracut UCT ultramicrotome (Leica, Vienna, Austria) and examined in a Hitachi HT 7700 (Hitachi, Tokyo, Japan) at 80 kV. Digital images were taken with a Veleta camera (Olympus Soft Imaging Solutions, GmbH, Münster, Germany).

Copyright and License information:  ©2016 Einarsson et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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