mRNA-LNP were thawed and freshly diluted on ice in Dulbecco’s Phosphate Buffered Saline (PBS) prior to each experiment. Mice were either 10–12 week-old Bl6/C57 female for the liver injury models or 7–8 week-old Balb/c female (purchased from the Jackson Laboratory, Bar Harbor, ME) to study the kinetics of expression and identity of cell types transfected in vivo using luciferase- and eGFP mRNA-LNP at homeostasis. Mice were administered 100 μl of diluted mRNA-LNP-encoding luciferase (5 μg), eGFP (10 μg), HGF (10 μg), Poly(C) (10 μg), or combination HGF (5 μg) + EGF (5 μg) intravenously by either retro-orbital or tail vein injections using 1/2cc lo-dose insulin syringes (EXELINT).
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