In vivo administration of mRNA-LNP

FR Fatima Rizvi
EE Elissa Everton
AS Anna R. Smith
HL Hua Liu
EO Elizabeth Osota
MB Mitchell Beattie
YT Ying Tam
NP Norbert Pardi
DW Drew Weissman
VG Valerie Gouon-Evans
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mRNA-LNP were thawed and freshly diluted on ice in Dulbecco’s Phosphate Buffered Saline (PBS) prior to each experiment. Mice were either 10–12 week-old Bl6/C57 female for the liver injury models or 7–8 week-old Balb/c female (purchased from the Jackson Laboratory, Bar Harbor, ME) to study the kinetics of expression and identity of cell types transfected in vivo using luciferase- and eGFP mRNA-LNP at homeostasis. Mice were administered 100 μl of diluted mRNA-LNP-encoding luciferase (5 μg), eGFP (10 μg), HGF (10 μg), Poly(C) (10 μg), or combination HGF (5 μg) + EGF (5 μg) intravenously by either retro-orbital or tail vein injections using 1/2cc lo-dose insulin syringes (EXELINT).

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