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ANS fluorescence experiment

ML Meng-Hsuan Lin
CC Chao-Cheng Cho
YC Yi-Chih Chiu
CC Chia-Yu Chien
YH Yi-Ping Huang
CC Chi-Fon Chang
CH Chun-Hua Hsu
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8-Anilinonaphthalene-1-sulfonic acid (ANS) is a fluorescence probe to detect the exposure of protein hydrophobic core76. Fresh 10 mM ANS was prepared by dissolving in methanol and added to the protein solution to a final concentration of 40 μM. The protein solutions with a final concentration of 0.2 mM macro domain were prepared with or without 0.15 mM guanidine hydrochloride. A sample without protein and guanidine hydrochloride was a control. The excitation wavelength was 360 nm, and spectra were respectively recorded in a region of 400–600 nm by temperature 298 or 308 K.

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