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POC [poly (1,8 octamethylene citrate)] scaffold synthesis

MB Matthew I. Bury
NF Natalie J. Fuller
RS Renea M. Sturm
RR Rebecca R. Rabizadeh
BN Bonnie G. Nolan
MB Milica Barac
SE Sonia S. Edassery
YC Yvonne Y. Chan
AS Arun K. Sharma
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POC scaffold synthesis was undertaken as previously described in detail29. Briefly, equimolar amounts of citric acid and 1,8 octanediol (Sigma Aldrich, St. Louis, MO) were combined then melted at 160–165 °C with stirring followed by a reduction in temperature to 140 °C for 25 min for pre-polymer production. The pre-polymer was dissolved in 100% ethanol to create a 30% w/v solution. This solution was distributed into a flat glass dish and polymerized for 7 days at 55 °C in air. POC scaffolds were removed from their glass molds and placed in DMEM (Lonza, Inc. Walkersville, MD) to remove any unreacted monomer. Media was changed every 6 h for 24 h. The dimensions of the POC scaffold used in vivo were 0.5 cm length × 0.5 cm width × 0.05 cm height.

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