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PLE (1.25 mg/mL) was incubated with Vero E6 cells in six-well plates (1 mL/well) between −3 h post-infection (p.i.) and 0 h p.i., between −1 h p.i. and 0 h p.i., between 0 h p.i. and 24 h p.i., and between −3 h and 24 h p.i. at 37 °C. The virus was absorbed to cells between −1 h p.i. and 0 h p.i. at an MOI of 0.01. Cell lysates from each treatment were harvested at 24 h p.i. for quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analyses.
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