Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Repeat Sequencing Analysis
Request a Protocol
Ask a
question
Favorite
MISA4 was used to identify SSRs in each species with the minimum numbers of repeats set to 8, 5, 3, 3, 3, and 3 for mono-, di-, tri-, tetra-, penta-, and hexanucleotides, respectively (Thiel et al., 2003). The long repetitive sequences containing forward, palindromic, reverse, and complementary repeats were analyzed using the software REPuter5 with a 30 bp minimum repeat size and a Hamming distance of 3 (Kurtz et al., 2001).
Copyright and License information: ©2021 Zhao, Li, Quan, Yang, Zhang, Liao and Lan.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
