Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Sequence annotation and genome comparison

PL Ping Li
QL Qiqiang Liang
WL Wugao Liu
BZ Beiwen Zheng
LL Lizhang Liu
WW Wei Wang
ZX Zhijiang Xu
MH Man Huang
YF Youjun Feng
request Request a Protocol
ask Ask a question
Favorite

Open reading frames (ORFs) were predicted using RAST (rapid annotation using subsystem technology, http://rast.nmpdr.org) combined with BLASTP/BLASTN searches. The plasmid maps were generated using GenomeVx (http://wolfe.ucd.ie/GenomeVx/). The comparison of circular plasmid map was given with the BLAST Ring Image Generator (version 0.95) [49], whereas linear alignments of multiple genomic loci were conducted using Easyfig [50]. Plasmid incompatibility typing were identified by PlasmidFinder 1.3 (https://cge.cbs.dtu.dk/services/PlasmidFinder-1.3/). Antibiotic resistance genes were detected by ResFinder 3.1 (https://cge.cbs.dtu.dk/services/ResFinder/).

Copyright and License information:  ©2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A