Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Acetylcholinesterase activity (AChE) assay
Ask a
question
Favorite
The AChE activity in SK-N-SH cells was assessed following to Ellman's method. First, the cell lysate was preincubated with 3 mM DTNB. Then, the acetylthiocholine iodide was mixed in the reaction and immediately read the absorbance at 405 nm for a regular interval of 2 min using a spectrophotometer (Synergy HT microplate reader, Biotek, VT, USA).
Copyright and License information: ©2021 Journal of Advanced Pharmaceutical Technology & Research
This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
