3.6. Carrageenan Induced Paw Edema and Anti-Inflammatory Evaluation

Carrageenan was prepared at 1% in D.W. and injected (0.1 mL) into the rat’s right hind paw (sub plantar). As a result, an acute inflammation manifested as edema (acute inflammation) was induced [45].

The rats were divided into five groups, five rats each. Three first groups were controls (normal control group without edema induction given orally 2 mL of D.W., negative control group with induced edema given orally 2 mL of D.W., and positive control group given orally diclofenac at the dose of 15 mg/kg) and the last two groups were rats with induced edema given orally PCS at the doses of 25 and 50 mg/kg.

After 12h of food deprivation and 1 h before the starting of the test, all groups received their treatments. At “0” h, the paw thickness was measured before the injection and after 1, 2, 3, 4, and 6 h after.

Anti-inflammatory activity was measured as the percentage reduction in edema level when the drug was present, relative to control.