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2.9. Scorecard Assay of In Vitro iPSC Differentiation

LK Laurence Klipfel
MC Marie Cordonnier
LT Léa Thiébault
EC Emmanuelle Clérin
FB Frédéric Blond
GM Géraldine Millet-Puel
SM Saddek Mohand-Saïd
OG Olivier Goureau
JS José-Alain Sahel
EN Emeline F. Nandrot
TL Thierry Léveillard
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The in vitro differentiation potential of iPSCs was assessed by their spontaneous differentiation into embryoid bodies (EBs). iPSCs were cultured in ultra-low adherent 6-well plates (Corning) for EB formation in Essential 6™ Medium. The medium was changed every 2–3 days, until day 20, when total RNA was extracted from EBs. Scorecard assay was performed by Thermo-Fisher Scientific on day 20-EBs compared to the corresponding iPSC clones. Scorecard values correspond to algorithm scores for the samples, showing upregulation or downregulation of the pluripotent (self-renewal), ectoderm, mesoderm and endoderm markers relative to a reference set of nine undifferentiated pluripotent stem cell lines.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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