2.2. Defining an rRT-PCR CT-Value Range for Potential Infectious Samples

Three quantitative comparison samples containing 10⁵, 10⁶, and 10⁷ SARS-CoV-2 (BetaCoV/Munich/ChVir984/2020) RNA copies/mL were used to generate a standard curve and to calculate the viral RNA copies/mL (Table S1 and Figure S1). The comparison samples were provided by INSTAND e.V. In total, 10 aliquots of each suspension were tested on two different days (5 aliquots/day) to verify the intra- and inter-assay reproducibility.