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4.6. Transfection of siRNA into Human Cardiac Fibroblast

PC Pao-Huan Chen
CC Cheng-Chih Chung
YL Yuan-Feng Lin
YK Yu-Hsun Kao
YC Yi-Jen Chen
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Human cardiac fibroblasts were transfected with 20 nM of either Orai1 siRNA (#s39560, Thermo Fisher Scientific, Loughborough, UK) or negative control siRNA (#4390843, Thermo Fisher Scientific, Loughborough, UK) using Lipofectamine RNAiMax Transfection Reagent (#13778150, Thermo Fisher Scientific, Loughborough, UK) for 48 h. The protein knockdown efficacy of Orai1 was assessed 48 h after siRNA transfection.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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