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IMVic (Indole, Methyl Red, Voges–Proskauer, and Citrate Utilization) and Indole Tests
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Pure culture of an isolate was inoculated in the tube of Tryptone Broth (M463, HiMedia, India) and incubated at 37°C for 24–48 h. Five drops of Kovac’s indole reagent (R008, HiMedia, India) was added directly onto the cultured tube. A positive indole test was indicated by the formation of pink to red color (cherry red ring) in the reagent layer on top of the medium. A negative test was indicated by yellow color on top of the reagent layer (MacWilliams, 2012).
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