4.5.1. alamarBlueTM Assay

To evaluate cell viability after printing, alamarBlue^TM assay was used according to the manufacturer’s recommendations (Thermo Fisher Scientific, Waltham, MA, USA). AlamarBlue^TM was diluted 1:10 in DMEM containing 10% FCS (AB medium). The medium was removed and each construct was incubated in 500 μL AB medium for 4 h at 37 °C and 5% CO₂. After the incubation period, 4 × 100 μL of each well was transferred to a 96-well plate. Fluorescence was measured in a plate reader with the following wavelength filter settings—540 nm for excitation and 590 nm for emission. AB medium without any cells served as a blank measurement. Two bioprinted constructs per condition were analyzed and measured in technical quadruplicates.